Hotspot

ndhF-rpl32

SSC · 104765-105709 · 945 bp

Back to Littledalea

Pi 0.000529
MarkerSeek score 46.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
945
variable_sites
1
pi
0.000529
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TCCAAATATTTTTACGAATACGCT 55.0 29.2 58.1 55.3 1048-1056 bp 1.0 40.7
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTGACATTGAAGTACGTTTTT 55.0 28.6 58.1 52.2 1021-1029 bp 1.0 40.7
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTTGACATTGAAGTACGTTTT 55.0 28.6 58.1 52.2 1022-1030 bp 1.0 40.7
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTGACATTGAAGTACGTTT 55.0 28.6 58.1 52.2 1023-1031 bp 1.0 40.7
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTTGACATTGAAGTACGTT 55.0 28.6 58.1 52.2 1024-1032 bp 1.0 40.7