Hotspot

petA-psbJ

LSC · 61058-61874 · 817 bp

Back to Littledalea

Pi 0.000612

MarkerSeek score 45.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 1
pi: 0.000612
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GCTGGTATTCCTGTGATTGGT	50.0	47.6	58.0	58.0	1011 bp	1.0	71.7
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	GCTGGTATTCCTGTGATTGGT	50.0	47.6	57.9	58.0	994 bp	1.0	71.6
petA-psbJ_p3	TGTTCTTCTTCGCATCCGTT	GCTGGTATTCCTGTGATTGGT	45.0	47.6	57.8	58.0	987 bp	1.0	70.7
petA-psbJ_p4	TTGTTCTTCTTCGCATCCGT	GCTGGTATTCCTGTGATTGGT	45.0	47.6	57.8	58.0	988 bp	1.0	70.7
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	GCTGGTATTCCTGTGATTGGT	50.0	47.6	58.2	58.0	1008 bp	1.0	70.6