Hotspot

atpB-rbcL

LSC · 54277-55059 · 783 bp

Back to Littledalea

Pi 0.000639

MarkerSeek score 45.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 783
variable_sites: 1
pi: 0.000639
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGGAAAAGTGACATCCAGCA	GCTTGAAATCCAACACCTGC	50.0	50.0	58.1	57.9	935-943 bp	1.0	78.8
atpB-rbcL_p2	GGGGGAAAAGTGACATCCAG	GCTTGAAATCCAACACCTGC	55.0	50.0	58.2	57.9	937-945 bp	1.0	78.2
atpB-rbcL_p3	CCAGCACGGGTCCAATAATT	GCTTGAAATCCAACACCTGC	50.0	50.0	58.2	57.9	921-929 bp	1.0	77.6
atpB-rbcL_p4	GAAAAGTGACATCCAGCACG	GCTTGAAATCCAACACCTGC	50.0	50.0	57.4	57.9	933-941 bp	1.0	74.7
atpB-rbcL_p5	TTCAATTGTGGAAACCCCGG	GCTTGAAATCCAACACCTGC	50.0	50.0	58.7	57.9	875-883 bp	1.0	74.2