Hotspot

ycf1

SSC · 128457-134024 · 5568 bp

Back to Litsea

Pi 0.00746
MarkerSeek score 51.00
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5568
variable_sites
267
pi
0.00746
unique_haplotype_count
27
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGCTTGGATTTGTGATAATTTGT AGAGTCAATGTACAACTTTTTCTTGA 30.4 30.8 55.1 57.5 234 bp 0.962963 46.5
ycf1_p2 AGCTTGGATTTGTGATAATTTGT GAGTCAATGTACAACTTTTTCTTGA 30.4 32.0 55.1 56.3 233 bp 0.962963 46.5
ycf1_p3 AGCTTGGATTTGTGATAATTTGT AGTCAATGTACAACTTTTTCTTGA 30.4 29.2 55.1 55.2 232 bp 0.962963 46.5
ycf1_p4 AGCTTGGATTTGTGATAATTTGT AGAGTCAATGTACAACTTTTTCTTG 30.4 32.0 55.1 56.2 234 bp 0.037037 1.5
ycf1_p5 AGCTTGGATTTGTGATAATTTGT AGAGTCAATGTACAACTTTTTCT 30.4 30.4 55.1 54.2 234 bp 0.037037 1.5