Hotspot

ndhF-rpl32

SSC · 115994-117211 · 1218 bp

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Pi 0.0152
MarkerSeek score 75.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1218
variable_sites
124
pi
0.0152
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.93
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAACAAGCAGAAGTCCCAA GTACGTTTCTTTGGAACCGC 50.0 50.0 58.0 57.7 1233-1343 bp 1.0 89.1
ndhF-rpl32_p2 AGCAGAAGTCCCAACCCTAT GTACGTTTCTTTGGAACCGC 50.0 50.0 58.0 57.7 1227-1337 bp 1.0 89.1
ndhF-rpl32_p3 AAGCAGAAGTCCCAACCCTA GTACGTTTCTTTGGAACCGC 50.0 50.0 58.3 57.7 1228-1338 bp 1.0 87.2
ndhF-rpl32_p4 GGAACAAGCAGAAGTCCCAA GGCCTGATATCCCCTTCCTT 50.0 55.0 58.0 58.5 1291-1401 bp 0.962963 84.7
ndhF-rpl32_p5 AGCAGAAGTCCCAACCCTAT GGCCTGATATCCCCTTCCTT 50.0 55.0 58.0 58.5 1285-1395 bp 0.962963 84.6