Hotspot

psbE-petL

LSC · 68308-69582 · 1275 bp

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Pi 0.00889
MarkerSeek score 72.10
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1275
variable_sites
86
pi
0.00889
unique_haplotype_count
24
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AAGCAGCCAGTAGAAAACCA 50.0 45.0 57.9 57.3 1422 bp 0.037037 35.8
psbE-petL_p2 CAACCCGCAATGAATAGGGA AGCAGCCAGTAGAAAACCAA 50.0 45.0 57.9 57.3 1421 bp 0.037037 35.8
psbE-petL_p3 ACGAATAACCAACCCGCAAT AAGCAGCCAGTAGAAAACCA 45.0 45.0 58.2 57.3 1431 bp 0.037037 34.9
psbE-petL_p4 ACGAATAACCAACCCGCAAT AGCAGCCAGTAGAAAACCAA 45.0 45.0 58.2 57.3 1430 bp 0.037037 34.9
psbE-petL_p5 TGCTGACGAATAACCAACCC AGCAGCCAGTAGAAAACCAA 50.0 45.0 58.2 57.3 1435 bp 0.037037 34.8