Hotspot

petA-psbJ

LSC · 66407-67538 · 1132 bp

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Pi 0.0189
MarkerSeek score 70.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1132
variable_sites
85
pi
0.0189
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCATCTGTTATCCTAGCACA GGCTCATATTCCGGATTGGG 47.6 55.0 57.5 58.2 1250-1331 bp 1.0 73.7
petA-psbJ_p2 TGGCATCTGTTATCCTAGCAC GGCTCATATTCCGGATTGGG 47.6 55.0 57.5 58.2 1251-1332 bp 1.0 73.7
petA-psbJ_p3 GGTTTGTTGTTCTTCTTGGCA GGCTCATATTCCGGATTGGG 42.9 55.0 57.5 58.2 1267-1348 bp 1.0 73.2
petA-psbJ_p4 TGAGAAGGTTCAATTGTCCGA GGCTCATATTCCGGATTGGG 42.9 55.0 57.2 58.2 1199-1280 bp 1.0 71.7
petA-psbJ_p5 AGGTTTGTTGTTCTTCTTGGC GGCTCATATTCCGGATTGGG 42.9 55.0 57.2 58.2 1268-1349 bp 1.0 71.0