Hotspot

trnS-GGA-rps4

LSC · 47620-47898 · 279 bp

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Pi 0.0159
MarkerSeek score 63.30
Primer available yes
Label trnS-GGA-rps4

Feature Metadata

label_name
trnS-GGA-rps4
feature_type
igs
region
LSC
length_bp
279
variable_sites
16
pi
0.0159
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-rps4_p1 TTCGAATCCCTCTCTTTCCG TGACTTTTGACTCATCCCAA 50.0 40.0 57.0 54.5 412-423 bp 1.0 49.3
trnS-GGA-rps4_p2 TTCGAATCCCTCTCTTTCCG TTGACTTTTGACTCATCCCA 50.0 40.0 57.0 54.5 413-424 bp 1.0 49.3
trnS-GGA-rps4_p3 GGGTTCGAATCCCTCTCTTT TGACTTTTGACTCATCCCAA 50.0 40.0 56.9 54.5 415-426 bp 1.0 48.3
trnS-GGA-rps4_p4 GATTATGTGGGAGAGATGGCC TGACTTTTGACTCATCCCAA 52.4 40.0 57.9 54.5 488-499 bp 0.962963 46.4
trnS-GGA-rps4_p5 GATTATGTGGGAGAGATGGCC TTGACTTTTGACTCATCCCA 52.4 40.0 57.9 54.5 489-500 bp 0.962963 46.4