Hotspot

psbM-trnD-GUC

LSC · 31414-32387 · 974 bp

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Pi 0.0117
MarkerSeek score 68.20
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
974
variable_sites
60
pi
0.0117
unique_haplotype_count
25
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAACGCAGTAGCA AGTTCAATCGGTCAGAGCAC 42.9 50.0 58.2 57.9 1059-1128 bp 1.0 77.9
psbM-trnD-GUC_p2 GAATGAACAACGCAGTAGCA AGTTCAATCGGTCAGAGCAC 45.0 50.0 56.7 57.9 1058-1127 bp 1.0 77.8
psbM-trnD-GUC_p3 AGAATGAACAACGCAGTAGCA ATGCTGTACACCCCATTTCC 42.9 50.0 58.2 57.9 1088-1157 bp 1.0 77.3
psbM-trnD-GUC_p4 GAATGAACAACGCAGTAGCA ATGCTGTACACCCCATTTCC 45.0 50.0 56.7 57.9 1087-1156 bp 1.0 77.2
psbM-trnD-GUC_p5 GAATGAACAACGCAGTAGCAA AGTTCAATCGGTCAGAGCAC 42.9 50.0 57.5 57.9 1058-1127 bp 1.0 75.5