primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	97	496	410-478	1.000000	90.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	97	496	407-475	0.962963	89.0
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	97	496	421-489	0.962963	88.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	97	496	420-488	0.962963	88.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	97	496	422-490	0.962963	88.9
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4386	5206	830-974	1.000000	86.3
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4386	5206	829-973	1.000000	86.3
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAAGACCATAGCCGCACTT	AAAAAGGCGCTCAACCTACA	20	20	45.0	45.0	57.8	58.0	0.0	7.6	0.224713	4386	5206	851-995	1.000000	86.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.3	58.0	5.3	7.6	0.307146	4386	5206	839-983	1.000000	85.4
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGCGCTCAACC	20	20	50.0	50.0	58.2	58.2	20.3	7.6	0.397463	4386	5206	833-977	1.000000	84.7
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6322	8196	1760-2014	1.000000	86.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GGGTACAAGAATCCACAGCC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.2	58.1	0.0	16.9	0.324664	6322	8196	1793-2047	1.000000	85.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	6322	8196	1764-2018	1.000000	85.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GGGTACAAGAATCCACAGCC	CTCGGAGGTTCGAATCCTTC	20	20	55.0	55.0	58.2	57.8	0.0	16.9	0.459915	6322	8196	1797-2051	1.000000	84.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGTACAAGAATCCACAGCCA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.566039	6322	8196	1792-2046	0.962963	81.9
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.204630	29093	30157	1158-1187	1.000000	84.2
trnC-GCA-petN_p2	trnC-GCA-petN	2	CTGCAAATCCTTTCTCCCCA	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.372001	29093	30157	1114-1143	1.000000	83.1
trnC-GCA-petN_p3	trnC-GCA-petN	3	TGCAAATCCTTTCTCCCCAG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.372001	29093	30157	1113-1142	1.000000	83.1
trnC-GCA-petN_p4	trnC-GCA-petN	4	ACTGCAAATCCTTTCTCCCC	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.373575	29093	30157	1115-1144	1.000000	83.1
trnC-GCA-petN_p5	trnC-GCA-petN	5	GCAAATCCTTTCTCCCCAGT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.373575	29093	30157	1112-1141	1.000000	83.1
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGTTGTTCATTCT	20	21	50.0	42.9	58.2	58.2	0.0	0.0	1.385036	30248	31308	1222-1235	1.000000	72.3
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGTTGTTCATTC	20	20	50.0	45.0	58.2	56.7	0.0	0.0	1.402106	30248	31308	1222-1235	1.000000	72.2
petN-psbM_p3	petN-psbM	3	GGGACACGATTCACATGGAT	TGCTACTGCGTTGTTCATTCT	20	21	50.0	42.9	57.7	58.2	0.0	0.0	1.565751	30248	31308	1243-1256	1.000000	70.9
petN-psbM_p4	petN-psbM	4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCGTTGTTCATTCT	20	21	50.0	42.9	57.6	58.2	0.0	0.0	1.584550	30248	31308	1161-1174	1.000000	70.9
petN-psbM_p5	petN-psbM	5	GGGACACGATTCACATGGAT	TGCTACTGCGTTGTTCATTC	20	20	50.0	45.0	57.7	56.7	0.0	0.0	1.582821	30248	31308	1243-1256	1.000000	70.7
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	21	20	42.9	50.0	58.2	57.9	0.0	0.0	1.319742	31414	32387	1059-1128	1.000000	77.9
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	GAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	56.7	57.9	0.0	0.0	1.336812	31414	32387	1058-1127	1.000000	77.8
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGAATGAACAACGCAGTAGCA	ATGCTGTACACCCCATTTCC	21	20	42.9	50.0	58.2	57.9	0.0	0.0	1.377563	31414	32387	1088-1157	1.000000	77.3
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	GAATGAACAACGCAGTAGCA	ATGCTGTACACCCCATTTCC	20	20	45.0	50.0	56.7	57.9	0.0	0.0	1.394634	31414	32387	1087-1156	1.000000	77.2
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	GAATGAACAACGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	21	20	42.9	50.0	57.5	57.9	0.0	0.0	1.622212	31414	32387	1058-1127	1.000000	75.5
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	32989	33708	823-833	1.000000	82.6
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	32989	33708	802-812	1.000000	82.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.327456	32989	33708	802-812	1.000000	82.3
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.345606	32989	33708	781-791	1.000000	82.2
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.349611	32989	33708	799-809	1.000000	82.1
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTCCTTAACC	20	20	55.0	55.0	58.1	57.9	7.6	0.0	0.169074	33781	35222	1575-1593	1.000000	82.5
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTCCTTAACC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.191228	33781	35222	1578-1596	1.000000	82.3
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	33781	35222	1583-1601	1.000000	81.3
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	33781	35222	1586-1604	1.000000	81.1
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	CGAAACGGTCCCTCCTTAAC	20	20	55.0	55.0	58.1	58.3	7.6	0.0	0.345672	33781	35222	1576-1594	1.000000	81.1
lhbA-trnG-GCC_p1	lhbA-trnG-GCC	1	TGCTTCTTCTGATGGTTGGT	CCTTACTTTACCATGGGCGG	20	20	45.0	55.0	57.0	58.3	0.0	18.1	1.281850	38471	38745	442-506	1.000000	76.8
lhbA-trnG-GCC_p2	lhbA-trnG-GCC	2	TGCTTCTTCTGATGGTTGGT	ACCTTACTTTACCATGGGCG	20	20	45.0	50.0	57.0	57.6	0.0	18.1	1.370712	38471	38745	443-507	1.000000	76.1
lhbA-trnG-GCC_p3	lhbA-trnG-GCC	3	TGCTTCTTCTGATGGTTGGT	GATCGAACCCGCATCTTCTC	20	20	45.0	55.0	57.0	58.5	0.0	0.0	1.464682	38471	38745	414-478	1.000000	75.5
lhbA-trnG-GCC_p4	lhbA-trnG-GCC	4	GCTTCTTCTGATGGTTGGTCA	CCTTACTTTACCATGGGCGG	21	20	47.6	55.0	58.2	58.3	0.0	18.1	1.541313	38471	38745	441-505	1.000000	74.7
lhbA-trnG-GCC_p5	lhbA-trnG-GCC	5	TGCTTCTTCTGATGGTTGGTC	CCTTACTTTACCATGGGCGG	21	20	47.6	55.0	58.2	58.3	0.0	18.1	1.541313	38471	38745	442-506	1.000000	74.7
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	TTCGAATCCCTCTCTTTCCG	TGACTTTTGACTCATCCCAA	20	20	50.0	40.0	57.0	54.5	0.0	0.0	4.484262	47620	47898	412-423	1.000000	49.3
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	TTCGAATCCCTCTCTTTCCG	TTGACTTTTGACTCATCCCA	20	20	50.0	40.0	57.0	54.5	0.0	0.0	4.484262	47620	47898	413-424	1.000000	49.3
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	GGGTTCGAATCCCTCTCTTT	TGACTTTTGACTCATCCCAA	20	20	50.0	40.0	56.9	54.5	17.2	0.0	4.605290	47620	47898	415-426	1.000000	48.3
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	GATTATGTGGGAGAGATGGCC	TGACTTTTGACTCATCCCAA	21	20	52.4	40.0	57.9	54.5	0.0	0.0	4.587635	47620	47898	488-499	0.962963	46.4
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	GATTATGTGGGAGAGATGGCC	TTGACTTTTGACTCATCCCA	21	20	52.4	40.0	57.9	54.5	0.0	0.0	4.587635	47620	47898	489-500	0.962963	46.4
ycf4-cemA_p1	ycf4-cemA	1	AAAAAGCTGCTGAATTGGCC	AGATCCACCAGGGCAAAAAG	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.245495	63618	64531	1026-1049	1.000000	84.9
ycf4-cemA_p2	ycf4-cemA	2	AAAAAGCTGCTGAATTGGCC	GATCCACCAGGGCAAAAAGA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.251008	63618	64531	1025-1048	1.000000	84.9
ycf4-cemA_p3	ycf4-cemA	3	AAAAAGCTGCTGAATTGGCC	GAGATCCACCAGGGCAAAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.251008	63618	64531	1027-1050	1.000000	84.8
ycf4-cemA_p4	ycf4-cemA	4	AAAGCTGCTGAATTGGCCTA	AGATCCACCAGGGCAAAAAG	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.291785	63618	64531	1024-1047	1.000000	84.5
ycf4-cemA_p5	ycf4-cemA	5	AAAGCTGCTGAATTGGCCTA	GATCCACCAGGGCAAAAAGA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.297297	63618	64531	1023-1046	1.000000	84.5
petA-psbJ_p1	petA-psbJ	1	GGCATCTGTTATCCTAGCACA	GGCTCATATTCCGGATTGGG	21	20	47.6	55.0	57.5	58.2	0.0	4.1	1.649181	66407	67538	1250-1331	1.000000	73.7
petA-psbJ_p2	petA-psbJ	2	TGGCATCTGTTATCCTAGCAC	GGCTCATATTCCGGATTGGG	21	20	47.6	55.0	57.5	58.2	0.0	4.1	1.649181	66407	67538	1251-1332	1.000000	73.7
petA-psbJ_p3	petA-psbJ	3	GGTTTGTTGTTCTTCTTGGCA	GGCTCATATTCCGGATTGGG	21	20	42.9	55.0	57.5	58.2	0.0	4.1	1.692249	66407	67538	1267-1348	1.000000	73.2
petA-psbJ_p4	petA-psbJ	4	TGAGAAGGTTCAATTGTCCGA	GGCTCATATTCCGGATTGGG	21	20	42.9	55.0	57.2	58.2	5.1	4.1	1.936022	66407	67538	1199-1280	1.000000	71.7
petA-psbJ_p5	petA-psbJ	5	AGGTTTGTTGTTCTTCTTGGC	GGCTCATATTCCGGATTGGG	21	20	42.9	55.0	57.2	58.2	0.0	4.1	1.974729	66407	67538	1268-1349	1.000000	71.0
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCA	20	20	50.0	45.0	57.9	57.3	6.4	0.0	0.708281	68308	69582	1422-1422	0.037037	35.8
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AGCAGCCAGTAGAAAACCAA	20	20	50.0	45.0	57.9	57.3	6.4	0.0	0.708281	68308	69582	1421-1421	0.037037	35.8
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCA	20	20	45.0	45.0	58.2	57.3	0.0	0.0	0.827968	68308	69582	1431-1431	0.037037	34.9
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	AGCAGCCAGTAGAAAACCAA	20	20	45.0	45.0	58.2	57.3	0.0	0.0	0.827968	68308	69582	1430-1430	0.037037	34.9
psbE-petL_p5	psbE-petL	5	TGCTGACGAATAACCAACCC	AGCAGCCAGTAGAAAACCAA	20	20	50.0	45.0	58.2	57.3	0.0	0.0	0.840873	68308	69582	1435-1435	0.037037	34.8
ndhF-rpl32_p1	ndhF-rpl32	1	GGAACAAGCAGAAGTCCCAA	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.323995	115994	117211	1233-1343	1.000000	89.1
ndhF-rpl32_p2	ndhF-rpl32	2	AGCAGAAGTCCCAACCCTAT	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.332664	115994	117211	1227-1337	1.000000	89.1
ndhF-rpl32_p3	ndhF-rpl32	3	AAGCAGAAGTCCCAACCCTA	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.562944	115994	117211	1228-1338	1.000000	87.2
ndhF-rpl32_p4	ndhF-rpl32	4	GGAACAAGCAGAAGTCCCAA	GGCCTGATATCCCCTTCCTT	20	20	50.0	55.0	58.0	58.5	0.0	0.0	0.568384	115994	117211	1291-1401	0.962963	84.7
ndhF-rpl32_p5	ndhF-rpl32	5	AGCAGAAGTCCCAACCCTAT	GGCCTGATATCCCCTTCCTT	20	20	50.0	55.0	58.0	58.5	0.0	0.0	0.577053	115994	117211	1285-1395	0.962963	84.6
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TTTTTGTGCGACAAACAAGT	TCCTAAGAGCAGCGTGTCTA	20	20	35.0	50.0	55.2	57.9	12.1	0.0	2.967765	117386	118777	1458-1482	1.000000	66.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTTTTGTGCGACAAACAAGT	AGCAGCGTGTCTACCAATTT	20	20	35.0	45.0	55.2	57.8	12.1	0.0	3.028492	117386	118777	1451-1475	1.000000	66.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TTTTTGTGCGACAAACAAGT	CCTAAGAGCAGCGTGTCTAC	20	20	35.0	55.0	55.2	57.8	12.1	0.0	3.039783	117386	118777	1457-1481	1.000000	65.9
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTTTTGTGCGACAAACAAGT	CTAAGAGCAGCGTGTCTACC	20	20	35.0	55.0	55.2	57.8	12.1	0.0	3.039783	117386	118777	1456-1480	1.000000	65.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTTTTGTGCGACAAACAAGT	GAGCAGCGTGTCTACCAATT	20	20	35.0	50.0	55.2	58.3	12.1	0.0	3.116634	117386	118777	1452-1476	1.000000	65.3
ycf1_p1	ycf1	1	AGCTTGGATTTGTGATAATTTGT	AGAGTCAATGTACAACTTTTTCTTGA	23	26	30.4	30.8	55.1	57.5	0.0	0.0	12.407936	130428	130547	234-234	0.962963	46.5
ycf1_p2	ycf1	2	AGCTTGGATTTGTGATAATTTGT	GAGTCAATGTACAACTTTTTCTTGA	23	25	30.4	32.0	55.1	56.3	0.0	0.0	12.645281	130428	130547	233-233	0.962963	46.5
ycf1_p3	ycf1	3	AGCTTGGATTTGTGATAATTTGT	AGTCAATGTACAACTTTTTCTTGA	23	24	30.4	29.2	55.1	55.2	0.0	0.0	12.726373	130428	130547	232-232	0.962963	46.5
ycf1_p4	ycf1	4	AGCTTGGATTTGTGATAATTTGT	AGAGTCAATGTACAACTTTTTCTTG	23	25	30.4	32.0	55.1	56.2	0.0	0.0	12.653420	130428	130547	234-234	0.037037	1.5
ycf1_p5	ycf1	5	AGCTTGGATTTGTGATAATTTGT	AGAGTCAATGTACAACTTTTTCT	23	23	30.4	30.4	55.1	54.2	0.0	0.0	12.742858	130428	130547	234-234	0.037037	1.5