Hotspot

ndhF

SSC · 116415-118679 · 2265 bp

Back to Lithocarpus

Pi 0.00962

MarkerSeek score 54.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2265
variable_sites: 123
pi: 0.00962
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CAGAGTACCGTCACCCATTG	AAGAACTGGCGAATCCGAAA	55.0	45.0	58.0	57.8	2593-2753 bp	1.0	87.1
ndhF_p2	ACGAAGGGGACTAAACAGGA	AAGAACTGGCGAATCCGAAA	50.0	45.0	58.0	57.8	2484-2644 bp	1.0	87.1
ndhF_p3	CAGAGTACCGTCACCCATTG	AACACAAGGCTACAATCGCT	55.0	45.0	58.0	57.8	2754-2926 bp	0.964286	85.7
ndhF_p4	CAGAGTACCGTCACCCATTG	ACACAAGGCTACAATCGCTT	55.0	45.0	58.0	57.8	2753-2925 bp	0.964286	85.7
ndhF_p5	ACGAAGGGGACTAAACAGGA	ACACAAGGCTACAATCGCTT	50.0	45.0	58.0	57.8	2644-2816 bp	0.964286	85.6