Hotspot

ndhA

SSC · 126669-128806 · 2138 bp

Back to Lithocarpus

Pi 0.00597

MarkerSeek score 66.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2138
variable_sites: 95
pi: 0.00597
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCTTGTTTGAGGCAAGCCAT	TGGGAGAAGTTGATCGTTGA	45.0	45.0	58.0	56.5	2136-2229 bp	1.0	74.9
ndhA_p2	TCTTGTTTGAGGCAAGCCAT	TGGGAGAAGTTGATCGTTGAA	45.0	42.9	58.0	57.2	2136-2229 bp	1.0	72.9
ndhA_p3	TCTCTTGTTTGAGGCAAGCC	TGGGAGAAGTTGATCGTTGA	50.0	45.0	58.4	56.5	2138-2231 bp	1.0	71.7
ndhA_p4	TCTTGTTTGAGGCAAGCCAT	ATGGGAGAAGTTGATCGTTGA	45.0	42.9	58.0	57.0	2137-2230 bp	1.0	71.1
ndhA_p5	TCTCTTGTTTGAGGCAAGCC	TGGGAGAAGTTGATCGTTGAA	50.0	42.9	58.4	57.2	2138-2231 bp	1.0	69.7