Hotspot

ndhF-rpl32

SSC · 118680-119627 · 948 bp

Back to Lithocarpus

Pi 0.00764

MarkerSeek score 66.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 948
variable_sites: 56
pi: 0.00764
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCGAAGATTTCTTGTTGCC	ACCCAATACCCCTCCCTTTT	50.0	50.0	58.0	58.2	1125-1166 bp	0.964286	83.7
ndhF-rpl32_p2	GCCGAAGATTTCTTGTTGCC	CTACCCAATACCCCTCCCTT	50.0	55.0	58.0	57.8	1127-1168 bp	0.928571	82.2
ndhF-rpl32_p3	GAGTCCCGCTCCTAGTAACA	ACCCAATACCCCTCCCTTTT	55.0	50.0	58.2	58.2	1091-1132 bp	0.964286	81.9
ndhF-rpl32_p4	GAGTCCCGCTCCTAGTAACA	CTACCCAATACCCCTCCCTT	55.0	55.0	58.2	57.8	1093-1134 bp	0.928571	80.4
ndhF-rpl32_p5	CCGAAGATTTCTTGTTGCCG	CTACCCAATACCCCTCCCTT	50.0	55.0	57.8	57.8	1126-1167 bp	0.928571	80.4