Hotspot

petA-psbJ

LSC · 68570-69592 · 1023 bp

Back to Lithocarpus

Pi 0.00567

MarkerSeek score 69.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1023
variable_sites: 34
pi: 0.00567
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	1158-1216 bp	1.0	81.2
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1155-1213 bp	1.0	80.1
petA-psbJ_p3	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1153-1211 bp	1.0	78.7
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	AGGCACTGTAACGGGTATTC	50.0	50.0	58.0	57.3	1217-1275 bp	1.0	78.4
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	GGCACTGTAACGGGTATTCT	50.0	50.0	58.0	57.3	1216-1274 bp	1.0	78.4