Hotspot

rbcL-accD

LSC · 62035-62642 · 608 bp

Back to Lithocarpus

Pi 0.0165

MarkerSeek score 70.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 608
variable_sites: 43
pi: 0.0165
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTGCTAAATGGAGTCC	AACATCGAGTTGAACCACCC	55.0	50.0	58.0	58.1	766-785 bp	1.0	85.4
rbcL-accD_p2	GAGGCTGCTAAATGGAGTCC	CATCGAGTTGAACCACCCTT	55.0	50.0	58.0	57.8	764-783 bp	1.0	84.8
rbcL-accD_p3	AATGGAGTCCTGAGCTAGCT	AACATCGAGTTGAACCACCC	50.0	50.0	57.8	58.1	756-775 bp	0.964286	82.9
rbcL-accD_p4	AATGGAGTCCTGAGCTAGCT	CATCGAGTTGAACCACCCTT	50.0	50.0	57.8	57.8	754-773 bp	0.964286	82.3
rbcL-accD_p5	TTCGTGAGGCTGCTAAATGG	AACATCGAGTTGAACCACCC	50.0	50.0	58.3	58.1	771-790 bp	0.964286	82.0