Hotspot

trnF-GAA-ndhJ

LSC · 53672-54519 · 848 bp

Back to Lithocarpus

Pi 0.0126

MarkerSeek score 69.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 848
variable_sites: 47
pi: 0.0126
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	983-1033 bp	1.0	87.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	947-997 bp	1.0	86.9
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	971-1021 bp	1.0	86.1
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	ATGCCCGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	992-1042 bp	1.0	84.9
trnF-GAA-ndhJ_p5	CGGATAATGGTCGGGATAGC	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.7	58.1	995-1045 bp	0.964286	83.2