Hotspot

petN-psbM

LSC · 31562-32542 · 981 bp

Back to Lithocarpus

Pi 0.00553

MarkerSeek score 67.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 981
variable_sites: 36
pi: 0.00553
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGTTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.3	57.6	1103-1151 bp	0.928571	70.0
petN-psbM_p2	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.9	57.6	1094-1142 bp	1.0	68.2
petN-psbM_p3	ATGTTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTC	50.0	45.0	58.3	56.1	1103-1151 bp	0.928571	65.8
petN-psbM_p4	ATGTTAAGTCTCGCTTGGGC	GCACTGTTCATTCTAGTTCCG	50.0	47.6	58.3	57.0	1096-1144 bp	0.928571	65.3
petN-psbM_p5	ATGTTAAGTCTCGCTTGGGC	TCATTCTAGTTCCGACTGCT	50.0	45.0	58.3	56.0	1089-1137 bp	0.928571	65.2