Hotspot

rpoB-trnC-GCA

LSC · 29169-30444 · 1276 bp

Back to Lithocarpus

Pi 0.00779

MarkerSeek score 61.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1276
variable_sites: 77
pi: 0.00779
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTCTACCCCCAAGC	CTGGGGTAGGGGTTACAGAA	55.0	55.0	57.9	58.0	1841-1976 bp	0.964286	85.7
rpoB-trnC-GCA_p2	GATACCGGGGCTTTGCAATA	CTGGGGTAGGGGTTACAGAA	50.0	55.0	58.0	58.0	2215-2350 bp	0.964286	85.0
rpoB-trnC-GCA_p3	GATACCGGGGCTTTGCAATA	TTTAGAGAACAGAACCGCCG	50.0	50.0	58.0	57.9	1859-1993 bp	0.964286	84.5
rpoB-trnC-GCA_p4	CCGCCTACACAAGCAAATTG	CTGGGGTAGGGGTTACAGAA	50.0	55.0	58.0	58.0	2523-2658 bp	0.964286	84.4
rpoB-trnC-GCA_p5	CCGCCTACACAAGCAAATTG	TTTAGAGAACAGAACCGCCG	50.0	50.0	58.0	57.9	2167-2301 bp	0.964286	83.9