Hotspot

trnK-UUU-rps16

LSC · 4430-5494 · 1065 bp

Back to Lithocarpus

Pi 0.0136

MarkerSeek score 63.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1065
variable_sites: 77
pi: 0.0136
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCCCCACACTCTAATGGAGA	CTCTTCGGAAAGTGGGCTTT	55.0	50.0	58.1	58.1	1628-1726 bp	1.0	87.5
trnK-UUU-rps16_p2	AAGACTGATCGACGGACTCT	CTCTTCGGAAAGTGGGCTTT	50.0	50.0	57.9	58.1	1805-1903 bp	1.0	87.2
trnK-UUU-rps16_p3	AGACTGATCGACGGACTCTT	CTCTTCGGAAAGTGGGCTTT	50.0	50.0	57.9	58.1	1804-1902 bp	1.0	87.2
trnK-UUU-rps16_p4	AAGTTTTGAACCGATCCGCT	CTCTTCGGAAAGTGGGCTTT	45.0	50.0	58.1	58.1	1370-1466 bp	0.964286	85.6
trnK-UUU-rps16_p5	GATCGACGGACTCTTTAGCC	CTCTTCGGAAAGTGGGCTTT	55.0	50.0	57.9	58.1	1851-1897 bp	0.75	76.4