Hotspot

trnH-GUG-psbA

LSC · 92-537 · 446 bp

Back to Lithocarpus

Pi 0.0283
MarkerSeek score 66.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
446
variable_sites
53
pi
0.0283
unique_haplotype_count
22
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CGTGCTAACCTTGGTATGGA 55.0 50.0 58.1 57.3 527-597 bp 1.0 85.2
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TCGTGCTAACCTTGGTATGG 55.0 50.0 58.1 57.3 528-598 bp 1.0 85.2
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CGTGCTAACCTTGGTATGGA 50.0 50.0 57.9 57.3 541-611 bp 1.0 85.0
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA TCGTGCTAACCTTGGTATGG 50.0 50.0 57.9 57.3 542-612 bp 1.0 85.0
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC CGTGCTAACCTTGGTATGGA 50.0 50.0 57.9 57.3 540-610 bp 1.0 85.0