Hotspot

rpoC2

LSC · 16795-20952 · 4158 bp

Back to Liriope

Pi 0.000441

MarkerSeek score 36.60

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4158
variable_sites: 3
pi: 0.000441
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ACTACGGGTCGAACCAAAAC	AAGAAGCCGAGTAGGTGGAT	50.0	50.0	58.1	58.1	249 bp	1.0	78.9
rpoC2_p2	ACTACGGGTCGAACCAAAAC	AGAAGCCGAGTAGGTGGATT	50.0	50.0	58.1	58.1	248 bp	1.0	78.9
rpoC2_p3	CTACGGGTCGAACCAAAACA	AAGAAGCCGAGTAGGTGGAT	50.0	50.0	58.1	58.1	248 bp	1.0	78.9
rpoC2_p4	CTACGGGTCGAACCAAAACA	AGAAGCCGAGTAGGTGGATT	50.0	50.0	58.1	58.1	247 bp	1.0	78.9
rpoC2_p5	GACTACGGGTCGAACCAAAA	AGAAGCCGAGTAGGTGGATT	50.0	50.0	57.8	58.1	249 bp	1.0	78.7