Hotspot

cemA

LSC · 62177-62914 · 738 bp

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Pi 0

MarkerSeek score 49.50

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 738
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TCCTTCTGGATCTAAGGAGTCA	TGCTTTCTTTATGCGCGAAG	45.5	45.0	57.4	57.5	910-912 bp	1.0	55.1
cemA_p2	TCCTTCTGGATCTAAGGAGTCA	GCTTTCTTTATGCGCGAAGA	45.5	45.0	57.4	57.2	909-911 bp	1.0	52.8
cemA_p3	TCCTTCTGGATCTAAGGAGTCAA	TGCTTTCTTTATGCGCGAAG	43.5	45.0	58.1	57.5	910-912 bp	1.0	51.6
cemA_p4	CCTTCTGGATCTAAGGAGTCA	TGCTTTCTTTATGCGCGAAG	47.6	45.0	55.9	57.5	909-911 bp	1.0	51.0
cemA_p5	TCCTTCTGGATCTAAGGAGTCA	ATGCTTTCTTTATGCGCGAAG	45.5	42.9	57.4	57.9	911-913 bp	1.0	50.8