Hotspot

trnN-GUU-ndhF

IRb · 110696-111839 · 1144 bp

Back to Liriope

Pi 0.00175

MarkerSeek score 41.40

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1144
variable_sites: 3
pi: 0.00175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	AATATATGGGAGGCGGACGA	50.0	50.0	58.2	58.1	1301-1302 bp	1.0	78.6
trnN-GUU-ndhF_p2	AATCAGTTAACAGCCGACCG	AATATATGGGAGGCGGACGA	50.0	50.0	58.3	58.1	1282-1283 bp	1.0	77.6
trnN-GUU-ndhF_p3	CCCCAAGTAGGATTCGAACC	AATATATGGGAGGCGGACGA	55.0	50.0	57.7	58.1	1309-1310 bp	1.0	77.2
trnN-GUU-ndhF_p4	AGGATTCGAACCTACGACCA	ATATATGGGAGGCGGACGAG	50.0	55.0	58.2	58.5	1300-1301 bp	1.0	74.9
trnN-GUU-ndhF_p5	CGAACCTACGACCAATCAGT	AATATATGGGAGGCGGACGA	50.0	50.0	57.4	58.1	1295-1296 bp	1.0	74.6