Hotspot

ycf4-cemA

LSC · 61375-62176 · 802 bp

Back to Liriope

Pi 0.00249

MarkerSeek score 37.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 3
pi: 0.00249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGACTCCGCGAGAAATTGAG	ATGAGAGAGAGACCCACCAG	50.0	55.0	58.0	57.8	992-994 bp	1.0	79.1
ycf4-cemA_p2	TGAGAATTTGACTCCGCGAG	ATGAGAGAGAGACCCACCAG	50.0	55.0	58.0	57.8	1000-1002 bp	1.0	79.1
ycf4-cemA_p3	TGCGCGTACCAATTGAAGTA	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.9	57.8	944-946 bp	1.0	78.5
ycf4-cemA_p4	CTTGCGCGTACCAATTGAAG	ATGAGAGAGAGACCCACCAG	50.0	55.0	58.1	57.8	946-948 bp	1.0	78.2
ycf4-cemA_p5	AAAAAGCTGCTGAATTGGCC	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.8	57.8	971-973 bp	1.0	77.8