Hotspot

trnT-UGU-trnL-UAA

LSC · 46557-47566 · 1010 bp

Back to Liriope

Pi 0.00066

MarkerSeek score 52.50

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1010
variable_sites: 1
pi: 0.00066
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	641-1093 bp	1.0	82.1
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	649-1101 bp	1.0	81.6
trnT-UGU-trnL-UAA_p3	AAATGCGATGCTCTAACCCC	ACCAATTTCGCCATATCCCC	50.0	50.0	58.3	58.0	613-1065 bp	1.0	81.5
trnT-UGU-trnL-UAA_p4	AAATGCGATGCTCTAACCCC	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.3	58.1	621-1073 bp	1.0	81.0
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	ATCCAATCAAGTCCGTAGCG	50.0	50.0	57.8	57.8	664-1116 bp	1.0	80.4