Hotspot

ndhF

SSC · 114215-116437 · 2223 bp

Back to Liriodendron

Pi 0.0039

MarkerSeek score 32.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 13
pi: 0.0039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	GAGAAGAGCTGGTGAATCGG	50.0	55.0	58.0	58.1	2669-2672 bp	1.0	80.4
ndhF_p2	ACGAAGGGGACTAAACAGGA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	2665-2668 bp	1.0	80.1
ndhF_p3	TTTAGGCAGAATACCGTCGC	GAGAAGAGCTGGTGAATCGG	50.0	55.0	58.1	58.1	2784-2787 bp	1.0	80.0
ndhF_p4	TTTAGGCAGAATACCGTCGC	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.1	57.9	2780-2783 bp	1.0	79.7
ndhF_p5	ACGAAGGGGACTAAACAGGA	GAGCTGGTGAATCGGAAACA	50.0	50.0	58.0	58.2	2664-2667 bp	1.0	79.4