Hotspot

ccsA

SSC · 119174-120139 · 966 bp

Back to Liriodendron

Pi 0.00345

MarkerSeek score 38.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 5
pi: 0.00345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TGGCGGCATCCTCTAAAAAG	GGTTCTCAAAAACTCGCACA	50.0	45.0	58.2	56.9	1135 bp	1.0	69.3
ccsA_p2	TGGCGGCATCCTCTAAAAAG	TGGTTCTCAAAAACTCGCAC	50.0	45.0	58.2	56.9	1136 bp	1.0	69.3
ccsA_p3	TGGCGGCATCCTCTAAAAAG	AAACTCGCACAAACTTTCGT	50.0	40.0	58.2	56.8	1126 bp	1.0	68.8
ccsA_p4	TGGCGGCATCCTCTAAAAAG	AACTCGCACAAACTTTCGTT	50.0	40.0	58.2	56.8	1125 bp	1.0	68.8
ccsA_p5	TGGCGGCATCCTCTAAAAAG	GGTTCTCAAAAACTCGCACAA	50.0	42.9	58.2	57.6	1135 bp	1.0	67.0