Hotspot

ycf2-trnI-CAU

IRa · 157299-157366 · 68 bp

Back to Liriodendron

Pi 0.0196

MarkerSeek score 52.50

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 68
variable_sites: 2
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	TGTGAGTGAAAGATCCCACTG	GTTGGGCGCTTTAACCATTC	47.6	50.0	57.6	58.0	212 bp	1.0	69.4
ycf2-trnI-CAU_p2	TCCCACTGAATCGAATTTGGT	GTTGGGCGCTTTAACCATTC	42.9	50.0	57.6	58.0	199 bp	1.0	69.3
ycf2-trnI-CAU_p3	GTGAGTGAAAGATCCCACTGA	GTTGGGCGCTTTAACCATTC	47.6	50.0	57.3	58.0	211 bp	1.0	67.2
ycf2-trnI-CAU_p4	CCACTGAATCGAATTTGGTCC	GTTGGGCGCTTTAACCATTC	47.6	50.0	57.2	58.0	197 bp	1.0	66.6
ycf2-trnI-CAU_p5	CCCACTGAATCGAATTTGGTC	GTTGGGCGCTTTAACCATTC	47.6	50.0	57.2	58.0	198 bp	1.0	66.6