Hotspot

petA-psbJ

LSC · 66379-67552 · 1174 bp

Back to Liriodendron

Pi 0.0108

MarkerSeek score 53.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1174
variable_sites: 19
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	CTGTGATCGGTTCAATGGGT	45.0	50.0	58.2	57.9	1351 bp	1.0	79.3
petA-psbJ_p2	ACGTGTCCAAGGTTTGTTGT	TCCTGTGATCGGTTCAATGG	45.0	50.0	58.2	57.6	1353 bp	1.0	77.0
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	CCTGTGATCGGTTCAATGGG	45.0	55.0	58.2	58.6	1352 bp	1.0	75.3
petA-psbJ_p4	TGGCATCTGTTATTCTGGCA	CTGTGATCGGTTCAATGGGT	45.0	50.0	57.2	57.9	1325 bp	1.0	74.3
petA-psbJ_p5	CGTGTCCAAGGTTTGTTGTT	CTGTGATCGGTTCAATGGGT	45.0	50.0	57.1	57.9	1350 bp	1.0	73.2