Hotspot

atpH-atpI

LSC · 14837-15927 · 1091 bp

Back to Liriodendron

Pi 0.00672

MarkerSeek score 50.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1091
variable_sites: 11
pi: 0.00672
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1179-1191 bp	1.0	76.3
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CAAGCGGGATTCAAGCTCTT	45.0	50.0	58.0	58.5	1200-1212 bp	1.0	76.1
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1235-1247 bp	1.0	75.7
atpH-atpI_p4	GATACCTTCTACGGCTTGGC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.1	58.5	1264-1276 bp	1.0	75.5
atpH-atpI_p5	CGATACCTTCTACGGCTTGG	TTTTTGCAACTTTAGCCGCG	55.0	45.0	57.9	58.5	1265-1277 bp	1.0	75.5