Hotspot

atpF-atpH

LSC · 14099-14590 · 492 bp

Back to Liriodendron

Pi 0.00679

MarkerSeek score 66.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 492
variable_sites: 5
pi: 0.00679
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	AATTTACGGACTGGTCGTGG	50.0	50.0	57.9	57.9	587-616 bp	1.0	82.8
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.9	58.3	573-602 bp	1.0	81.5
atpF-atpH_p3	CCAGTAACCCAAGGAAACGA	AATTTACGGACTGGTCGTGG	50.0	50.0	57.4	57.9	560-589 bp	1.0	79.5
atpF-atpH_p4	CCAGTAACCCAAGGAAACGA	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.4	58.3	546-575 bp	1.0	78.1
atpF-atpH_p5	GGCCAGTAACCCAAGGAAAC	AATTTACGGACTGGTCGTGG	55.0	50.0	58.7	57.9	562-591 bp	1.0	78.0