Hotspot

rps16-trnQ-UUG

LSC · 6143-8063 · 1921 bp

Back to Liriodendron

Pi 0.00729
MarkerSeek score 59.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1921
variable_sites
21
pi
0.00729
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAAGAATCCACAGCCGATCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 2021-2023 bp 1.0 80.3
rps16-trnQ-UUG_p2 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 2011-2013 bp 1.0 80.3
rps16-trnQ-UUG_p3 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 2009-2011 bp 1.0 80.3
rps16-trnQ-UUG_p4 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 2017-2019 bp 1.0 80.2
rps16-trnQ-UUG_p5 GAATCCACAGCCGATCATGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 2018-2020 bp 1.0 80.2