Hotspot

trnH-GUG-psbA

LSC · 100-507 · 408 bp

Back to Liriodendron

Pi 0.0278

MarkerSeek score 45.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 408
variable_sites: 17
pi: 0.0278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TCACAATCCACTGCCTTGAG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.8	57.9	492 bp	1.0	80.9
trnH-GUG-psbA_p2	TCACAATCCACTGCCTTGAG	GTTGACAGTCAAGGTCGTGT	50.0	50.0	57.8	58.1	591 bp	1.0	80.4
trnH-GUG-psbA_p3	TCACAATCCACTGCCTTGAG	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.8	58.0	878 bp	1.0	79.8
trnH-GUG-psbA_p4	TCACAATCCACTGCCTTGAG	CAGTGCTATGCATGGTTCCT	50.0	50.0	57.8	58.0	877 bp	1.0	79.7
trnH-GUG-psbA_p5	ACAAAGGAATGATGGGCGAA	TCAGTGCTATGCATGGTTCC	45.0	50.0	57.8	58.0	928 bp	0.666667	64.6