primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	TCACAATCCACTGCCTTGAG	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.8	57.9	0.0	13.4	0.255492	100	507	492-492	1.000000	80.9
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	TCACAATCCACTGCCTTGAG	GTTGACAGTCAAGGTCGTGT	20	20	50.0	50.0	57.8	58.1	0.0	16.7	0.253661	100	507	591-591	1.000000	80.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	TCACAATCCACTGCCTTGAG	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.8	58.0	0.0	10.2	0.232280	100	507	878-878	1.000000	79.8
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	TCACAATCCACTGCCTTGAG	CAGTGCTATGCATGGTTCCT	20	20	50.0	50.0	57.8	58.0	0.0	10.2	0.238038	100	507	877-877	1.000000	79.7
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAAAGGAATGATGGGCGAA	TCAGTGCTATGCATGGTTCC	20	20	45.0	50.0	57.8	58.0	0.0	10.2	0.257062	100	507	928-928	0.666667	64.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CAAGAATCCACAGCCGATCA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.104521	6143	8063	2021-2023	1.000000	80.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.104521	6143	8063	2011-2013	1.000000	80.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.105939	6143	8063	2009-2011	1.000000	80.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AATCCACAGCCGATCATGTC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	6143	8063	2017-2019	1.000000	80.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GAATCCACAGCCGATCATGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	6143	8063	2018-2020	1.000000	80.2
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	AATCCTGGACGCGAGGAATA	TGGACTAAAGCGACGGATTG	20	20	50.0	50.0	58.6	58.0	17.8	0.0	0.594578	9168	9309	232-236	1.000000	77.6
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	ATCCTGGACGCGAGGAATAA	TGGACTAAAGCGACGGATTG	20	20	50.0	50.0	58.6	58.0	17.8	0.0	0.594578	9168	9309	231-235	1.000000	77.6
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	TAATCCTGGACGCGAGGAAT	TGGACTAAAGCGACGGATTG	20	20	50.0	50.0	58.6	58.0	17.8	0.0	0.594578	9168	9309	233-237	1.000000	77.6
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	AATCCTGGACGCGAGGAATA	GTGGACTAAAGCGACGGATT	20	20	50.0	50.0	58.6	58.0	17.8	0.0	0.595966	9168	9309	233-237	1.000000	77.6
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	ATCCTGGACGCGAGGAATAA	GTGGACTAAAGCGACGGATT	20	20	50.0	50.0	58.6	58.0	17.8	0.0	0.595966	9168	9309	232-236	1.000000	77.6
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	AATTTACGGACTGGTCGTGG	20	20	50.0	50.0	57.9	57.9	0.0	7.6	0.207263	14099	14590	587-616	1.000000	82.8
atpF-atpH_p2	atpF-atpH	2	ATTAAACCCGAAACTCCCGG	TCGTGGCATTAGCGCTTTTA	20	20	50.0	45.0	57.9	58.3	0.0	18.7	0.390245	14099	14590	573-602	1.000000	81.5
atpF-atpH_p3	atpF-atpH	3	CCAGTAACCCAAGGAAACGA	AATTTACGGACTGGTCGTGG	20	20	50.0	50.0	57.4	57.9	0.0	7.6	0.643320	14099	14590	560-589	1.000000	79.5
atpF-atpH_p4	atpF-atpH	4	CCAGTAACCCAAGGAAACGA	TCGTGGCATTAGCGCTTTTA	20	20	50.0	45.0	57.4	58.3	0.0	18.7	0.826303	14099	14590	546-575	1.000000	78.1
atpF-atpH_p5	atpF-atpH	5	GGCCAGTAACCCAAGGAAAC	AATTTACGGACTGGTCGTGG	20	20	55.0	50.0	58.7	57.9	0.0	7.6	0.834923	14099	14590	562-591	1.000000	78.0
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.0	58.5	1.4	0.0	0.556109	14837	15927	1179-1191	1.000000	76.3
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	CAAGCGGGATTCAAGCTCTT	20	20	45.0	50.0	58.0	58.5	1.4	5.7	0.581041	14837	15927	1200-1212	1.000000	76.1
atpH-atpI_p3	atpH-atpI	3	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	57.9	58.5	0.0	0.0	0.630002	14837	15927	1235-1247	1.000000	75.7
atpH-atpI_p4	atpH-atpI	4	GATACCTTCTACGGCTTGGC	TTTTTGCAACTTTAGCCGCG	20	20	55.0	45.0	58.1	58.5	0.0	0.0	0.650556	14837	15927	1264-1276	1.000000	75.5
atpH-atpI_p5	atpH-atpI	5	CGATACCTTCTACGGCTTGG	TTTTTGCAACTTTAGCCGCG	20	20	55.0	45.0	57.9	58.5	0.0	0.0	0.653387	14837	15927	1265-1277	1.000000	75.5
trnfM-CAU-rps14_p1	trnfM-CAU-rps14	1	GCTACCAAACTGCTCTACCC	CGTTGTTTTTCGACCGGAAG	20	20	55.0	50.0	58.0	58.0	0.0	9.5	0.038874	39402	39593	306-336	1.000000	83.9
trnfM-CAU-rps14_p2	trnfM-CAU-rps14	2	AAGGTTATGAGCCTTGCGAG	CGTTGTTTTTCGACCGGAAG	20	20	50.0	50.0	58.0	58.0	0.0	9.5	0.043124	39402	39593	325-355	1.000000	83.9
trnfM-CAU-rps14_p3	trnfM-CAU-rps14	3	GATGTTGCGGAGACAGGATT	CGTTGTTTTTCGACCGGAAG	20	20	50.0	50.0	58.0	58.0	0.0	9.5	0.043124	39402	39593	360-390	1.000000	83.9
trnfM-CAU-rps14_p4	trnfM-CAU-rps14	4	TTTGAACCCGTGACCTCAAG	CGTTGTTTTTCGACCGGAAG	20	20	50.0	50.0	58.0	58.0	0.0	9.5	0.051492	39402	39593	342-372	1.000000	83.8
trnfM-CAU-rps14_p5	trnfM-CAU-rps14	5	ACAGGATTTGAACCCGTGAC	CGTTGTTTTTCGACCGGAAG	20	20	50.0	50.0	58.1	58.0	0.0	9.5	0.121076	39402	39593	348-378	1.000000	83.3
rps14-psaB_p1	rps14-psaB	1	TGCCTTTTTCTCTCCCTCTG	ACCAGTGGCTCTTTCCATTG	20	20	50.0	50.0	57.1	58.1	0.0	9.8	0.951968	39897	40038	301-303	1.000000	73.7
rps14-psaB_p2	rps14-psaB	2	TGCCTTTTTCTCTCCCTCTG	CCAGTGGCTCTTTCCATTGT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.951968	39897	40038	300-302	1.000000	73.7
rps14-psaB_p3	rps14-psaB	3	TGCCTTTTTCTCTCCCTCTG	GCAGCTTTCTTGATTGCCTC	20	20	50.0	50.0	57.1	57.7	0.0	0.0	1.142029	39897	40038	225-227	1.000000	72.6
rps14-psaB_p4	rps14-psaB	4	TGCCTTTTTCTCTCCCTCTG	GCTCTTTCCATTGTGCAAGC	20	20	50.0	50.0	57.1	58.3	0.0	0.0	1.160666	39897	40038	294-296	1.000000	72.1
rps14-psaB_p5	rps14-psaB	5	TGCCTTTTTCTCTCCCTCTG	TGGCTCTTTCCATTGTGCAA	20	20	50.0	45.0	57.1	58.3	0.0	0.0	1.164927	39897	40038	296-298	1.000000	72.0
petA-psbJ_p1	petA-psbJ	1	ACGTGTCCAAGGTTTGTTGT	CTGTGATCGGTTCAATGGGT	20	20	45.0	50.0	58.2	57.9	0.0	0.0	0.284881	66379	67552	1351-1351	1.000000	79.3
petA-psbJ_p2	petA-psbJ	2	ACGTGTCCAAGGTTTGTTGT	TCCTGTGATCGGTTCAATGG	20	20	45.0	50.0	58.2	57.6	0.0	0.0	0.572604	66379	67552	1353-1353	1.000000	77.0
petA-psbJ_p3	petA-psbJ	3	ACGTGTCCAAGGTTTGTTGT	CCTGTGATCGGTTCAATGGG	20	20	45.0	55.0	58.2	58.6	0.0	0.0	0.788499	66379	67552	1352-1352	1.000000	75.3
petA-psbJ_p4	petA-psbJ	4	TGGCATCTGTTATTCTGGCA	CTGTGATCGGTTCAATGGGT	20	20	45.0	50.0	57.2	57.9	0.0	0.0	0.918847	66379	67552	1325-1325	1.000000	74.3
petA-psbJ_p5	petA-psbJ	5	CGTGTCCAAGGTTTGTTGTT	CTGTGATCGGTTCAATGGGT	20	20	45.0	50.0	57.1	57.9	0.0	0.0	1.045372	66379	67552	1350-1350	1.000000	73.2
petL-petG_p1	petL-petG	1	TGAGCAAGATACGACTTATTTGA	GATCACCGCGTCTGTATTGT	23	20	34.8	50.0	55.8	58.1	0.0	4.9	5.289338	69661	69837	300-300	1.000000	40.5
petL-petG_p2	petL-petG	2	TGAGCAAGATACGACTTATTTGA	TGATCACCGCGTCTGTATTG	23	20	34.8	50.0	55.8	58.1	0.0	4.9	5.290665	69661	69837	301-301	1.000000	40.5
petL-petG_p3	petL-petG	3	TGAGCAAGATACGACTTATTTGA	AAAGGTCCAACTGATCACCG	23	20	34.8	50.0	55.8	57.8	0.0	0.0	5.396913	69661	69837	312-312	1.000000	40.5
petL-petG_p4	petL-petG	4	TGAGCAAGATACGACTTATTTGA	CTGATCACCGCGTCTGTATT	23	20	34.8	50.0	55.8	57.8	0.0	4.9	5.421747	69661	69837	302-302	1.000000	40.5
petL-petG_p5	petL-petG	5	TGAGCAAGATACGACTTATTTG	GATCACCGCGTCTGTATTGT	22	20	36.4	50.0	54.3	58.1	0.0	4.9	5.741935	69661	69837	300-300	1.000000	40.5
petG-trnW-CCA_p1	petG-trnW-CCA	1	ACAATACAGACGCGGTGATC	AGAACGTGGGTCTCCAAAAC	20	20	50.0	50.0	58.1	58.0	12.5	0.0	0.114258	69952	70065	201-202	1.000000	81.5
petG-trnW-CCA_p2	petG-trnW-CCA	2	CAATACAGACGCGGTGATCA	AGAACGTGGGTCTCCAAAAC	20	20	50.0	50.0	58.1	58.0	12.5	0.0	0.115584	69952	70065	200-201	1.000000	81.5
petG-trnW-CCA_p3	petG-trnW-CCA	3	ACAATACAGACGCGGTGATC	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.1	58.0	12.5	2.6	0.093457	69952	70065	223-224	1.000000	81.4
petG-trnW-CCA_p4	petG-trnW-CCA	4	CAATACAGACGCGGTGATCA	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.1	58.0	12.5	2.6	0.094784	69952	70065	222-223	1.000000	81.4
petG-trnW-CCA_p5	petG-trnW-CCA	5	ACAATACAGACGCGGTGATC	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	58.1	57.9	12.5	1.4	0.166379	69952	70065	187-188	1.000000	81.2
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	TGTGAGTGAAAGATCCCACTG	20	21	50.0	47.6	58.0	57.6	7.6	15.1	1.416624	89830	89897	212-212	1.000000	69.4
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	TCCCACTGAATCGAATTTGGT	20	21	50.0	42.9	58.0	57.6	7.6	0.0	1.442506	89830	89897	199-199	1.000000	69.3
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	GTTGGGCGCTTTAACCATTC	GTGAGTGAAAGATCCCACTGA	20	21	50.0	47.6	58.0	57.3	7.6	15.1	1.694340	89830	89897	211-211	1.000000	67.2
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	GTTGGGCGCTTTAACCATTC	CCACTGAATCGAATTTGGTCC	20	21	50.0	47.6	58.0	57.2	7.6	0.0	1.774086	89830	89897	197-197	1.000000	66.6
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	GTTGGGCGCTTTAACCATTC	CCCACTGAATCGAATTTGGTC	20	21	50.0	47.6	58.0	57.2	7.6	0.0	1.774086	89830	89897	198-198	1.000000	66.6
ndhF_p1	ndhF	1	ACGAAGGGGACTAAACAGGA	GAGAAGAGCTGGTGAATCGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.086561	114215	116437	2669-2672	1.000000	80.4
ndhF_p2	ndhF	2	ACGAAGGGGACTAAACAGGA	AGAGCTGGTGAATCGGAAAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.120412	114215	116437	2665-2668	1.000000	80.1
ndhF_p3	ndhF	3	TTTAGGCAGAATACCGTCGC	GAGAAGAGCTGGTGAATCGG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.136186	114215	116437	2784-2787	1.000000	80.0
ndhF_p4	ndhF	4	TTTAGGCAGAATACCGTCGC	AGAGCTGGTGAATCGGAAAC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.170037	114215	116437	2780-2783	1.000000	79.7
ndhF_p5	ndhF	5	ACGAAGGGGACTAAACAGGA	GAGCTGGTGAATCGGAAACA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.211397	114215	116437	2664-2667	1.000000	79.4
ndhF-rpl32_p1	ndhF-rpl32	1	GGAACAAGCAGAAGTCCCAA	TTTACCGCTGCCCAATATCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.043084	116438	117573	1314-1314	1.000000	80.4
ndhF-rpl32_p2	ndhF-rpl32	2	CAGTCGGAACAAGCAGAAGT	TTTACCGCTGCCCAATATCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.153260	116438	117573	1319-1319	1.000000	79.6
ndhF-rpl32_p3	ndhF-rpl32	3	AGTCGGAACAAGCAGAAGTC	TTTACCGCTGCCCAATATCC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.180683	116438	117573	1318-1318	1.000000	79.3
ndhF-rpl32_p4	ndhF-rpl32	4	ACAAGCAGAAGTCCCAATCC	TTTACCGCTGCCCAATATCC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.230916	116438	117573	1311-1311	1.000000	78.9
ndhF-rpl32_p5	ndhF-rpl32	5	CTGGAAGTGGAACGAAAGGT	TTTACCGCTGCCCAATATCC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.280458	116438	117573	1277-1277	1.000000	78.6
ccsA_p1	ccsA	1	TGGCGGCATCCTCTAAAAAG	GGTTCTCAAAAACTCGCACA	20	20	50.0	45.0	58.2	56.9	8.0	0.0	1.376557	119174	120139	1135-1135	1.000000	69.3
ccsA_p2	ccsA	2	TGGCGGCATCCTCTAAAAAG	TGGTTCTCAAAAACTCGCAC	20	20	50.0	45.0	58.2	56.9	8.0	0.0	1.376557	119174	120139	1136-1136	1.000000	69.3
ccsA_p3	ccsA	3	TGGCGGCATCCTCTAAAAAG	AAACTCGCACAAACTTTCGT	20	20	50.0	40.0	58.2	56.8	8.0	0.0	1.446181	119174	120139	1126-1126	1.000000	68.8
ccsA_p4	ccsA	4	TGGCGGCATCCTCTAAAAAG	AACTCGCACAAACTTTCGTT	20	20	50.0	40.0	58.2	56.8	8.0	0.0	1.446181	119174	120139	1125-1125	1.000000	68.8
ccsA_p5	ccsA	5	TGGCGGCATCCTCTAAAAAG	GGTTCTCAAAAACTCGCACAA	20	21	50.0	42.9	58.2	57.6	8.0	0.0	1.664356	119174	120139	1135-1135	1.000000	67.0
ccsA-ndhD_p1	ccsA-ndhD	1	TGTGGCTTCCATGGGATTTC	TCCTGATTTCGTTCTCTCGC	20	20	50.0	50.0	58.1	57.7	13.0	0.0	0.428278	120140	120366	403-403	1.000000	78.2
ccsA-ndhD_p2	ccsA-ndhD	2	GTGGCTTCCATGGGATTTCT	TCCTGATTTCGTTCTCTCGC	20	20	50.0	50.0	57.9	57.7	13.0	0.0	0.429555	120140	120366	402-402	1.000000	78.2
ccsA-ndhD_p3	ccsA-ndhD	3	TGTGGCTTCCATGGGATTTC	CCTGATTTCGTTCTCTCGCT	20	20	50.0	50.0	58.1	57.7	13.0	0.0	0.434523	120140	120366	402-402	1.000000	78.1
ccsA-ndhD_p4	ccsA-ndhD	4	GTGGCTTCCATGGGATTTCT	CCTGATTTCGTTCTCTCGCT	20	20	50.0	50.0	57.9	57.7	13.0	0.0	0.435800	120140	120366	401-401	1.000000	78.1
ccsA-ndhD_p5	ccsA-ndhD	5	TTGTGGCTTCCATGGGATTT	TCCTGATTTCGTTCTCTCGC	20	20	45.0	50.0	57.7	57.7	13.0	0.0	0.603581	120140	120366	404-404	1.000000	76.8
ycf1_p1	ycf1	1	TGGAATCCTCTGTCCAAGTG	AGGCACAACTTGAGCAATGA	20	20	50.0	45.0	56.8	58.0	0.0	12.5	1.173194	132742	132861	229-229	1.000000	72.7
ycf1_p2	ycf1	2	TGGAATCCTCTGTCCAAGTGA	AGGCACAACTTGAGCAATGA	21	20	47.6	45.0	58.4	58.0	0.0	12.5	1.394596	132742	132861	229-229	1.000000	70.9
ycf1_p3	ycf1	3	GGAATCCTCTGTCCAAGTGA	AGGCACAACTTGAGCAATGA	20	20	50.0	45.0	56.5	58.0	0.0	12.5	1.467381	132742	132861	228-228	1.000000	70.4
ycf1_p4	ycf1	4	TGGAATCCTCTGTCCAAGTG	GGCACAACTTGAGCAATGAG	20	20	50.0	50.0	56.8	57.7	0.0	12.5	1.501577	132742	132861	228-228	1.000000	70.1
ycf1_p5	ycf1	5	ATGGAATCCTCTGTCCAAGTG	AGGCACAACTTGAGCAATGA	21	20	47.6	45.0	57.4	58.0	0.0	12.5	1.655961	132742	132861	230-230	1.000000	68.8
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	TGTGAGTGAAAGATCCCACTG	GTTGGGCGCTTTAACCATTC	21	20	47.6	50.0	57.6	58.0	15.1	7.6	1.416624	157299	157366	212-212	1.000000	69.4
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	TCCCACTGAATCGAATTTGGT	GTTGGGCGCTTTAACCATTC	21	20	42.9	50.0	57.6	58.0	0.0	7.6	1.442506	157299	157366	199-199	1.000000	69.3
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	GTGAGTGAAAGATCCCACTGA	GTTGGGCGCTTTAACCATTC	21	20	47.6	50.0	57.3	58.0	15.1	7.6	1.694340	157299	157366	211-211	1.000000	67.2
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	CCACTGAATCGAATTTGGTCC	GTTGGGCGCTTTAACCATTC	21	20	47.6	50.0	57.2	58.0	0.0	7.6	1.774086	157299	157366	197-197	1.000000	66.6
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	CCCACTGAATCGAATTTGGTC	GTTGGGCGCTTTAACCATTC	21	20	47.6	50.0	57.2	58.0	0.0	7.6	1.774086	157299	157366	198-198	1.000000	66.6