Hotspot

ycf1

SSC · 129592-135228 · 5637 bp

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Pi 0.00612

MarkerSeek score 34.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5637
variable_sites: 83
pi: 0.00612
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCTTTGATCATGTCTCGGC	GAAGCTCTAGCCAAAGGGTC	50.0	55.0	58.0	58.0	732-768 bp	1.0	81.9
ycf1_p2	ATATCCCAGGCTTCCGCTAT	GAAGCTCTAGCCAAAGGGTC	50.0	55.0	58.0	58.0	995-1031 bp	1.0	81.8
ycf1_p3	CTCTTCCTCCGCATAATCCG	GAAGCTCTAGCCAAAGGGTC	55.0	55.0	57.9	58.0	927-963 bp	1.0	81.6
ycf1_p4	CTTTGATCATGTCTCGGCGA	GAAGCTCTAGCCAAAGGGTC	50.0	55.0	58.1	58.0	730-766 bp	1.0	81.5
ycf1_p5	ACCTTTGATCATGTCTCGGC	CTTGAACGGACCCTATCGTG	50.0	55.0	58.0	58.1	625-661 bp	1.0	81.4