Hotspot

rpl36-infA

LSC · 84308-84434 · 127 bp

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Pi 0.0122

MarkerSeek score 46.00

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 4
pi: 0.0122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	ACTATAATTCGTCCCCGCCT	AGACTCCGCAACAAGGATTC	50.0	50.0	58.3	57.9	225-231 bp	1.0	80.4
rpl36-infA_p2	ACTATAATTCGTCCCCGCCT	CGCAACAAGGATTCGAAGGA	50.0	50.0	58.3	58.6	219-225 bp	1.0	76.8
rpl36-infA_p3	ACTATAATTCGTCCCCGCCT	CTCCGCAACAAGGATTCGAA	50.0	50.0	58.3	58.6	222-228 bp	1.0	76.8
rpl36-infA_p4	ACTATAATTCGTCCCCGCCT	TCCGCAACAAGGATTCGAAG	50.0	50.0	58.3	58.6	221-227 bp	1.0	76.8
rpl36-infA_p5	ACTATAATTCGTCCCCGCCT	TATGATTCAACCAGAGGGCG	50.0	50.0	58.3	57.4	255-261 bp	1.0	75.8