Hotspot

petA-psbJ

LSC · 67376-68447 · 1072 bp

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Pi 0.0104

MarkerSeek score 59.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1072
variable_sites: 25
pi: 0.0104
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGTCCGA	TGGTTCATATTCAGGATTGGGT	42.9	40.9	57.2	57.1	1127-1140 bp	1.0	47.3
petA-psbJ_p2	TGAGAAGGTTCAATTGTCCGA	ATTCAGGATTGGGTTCATCC	42.9	45.0	57.2	55.1	1119-1132 bp	1.0	46.8
petA-psbJ_p3	TGAGAAGGTTCAATTGTCCGAA	TGGTTCATATTCAGGATTGGGT	40.9	40.9	57.9	57.1	1127-1140 bp	1.0	44.8
petA-psbJ_p4	TTGAGAAGGTTCAATTGTCCGA	TGGTTCATATTCAGGATTGGGT	40.9	40.9	57.9	57.1	1128-1141 bp	1.0	44.8
petA-psbJ_p5	TGAGAAGGTTCAATTGTCCGA	TGGTTCATATTCAGGATTGGGTT	42.9	39.1	57.2	57.8	1127-1140 bp	1.0	44.7