Hotspot

ycf3-tRNA-GGA

LSC · 47669-48500 · 832 bp

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Pi 0.00828

MarkerSeek score 44.50

Primer available yes

Label ycf3-tRNA-GGA

Feature Metadata

label_name: ycf3-tRNA-GGA
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 15
pi: 0.00828
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-tRNA-GGA_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	918-926 bp	1.0	72.5
ycf3-tRNA-GGA_p2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	939-947 bp	1.0	72.0
ycf3-tRNA-GGA_p3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	889-897 bp	1.0	71.7
ycf3-tRNA-GGA_p4	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	919-927 bp	1.0	70.1
ycf3-tRNA-GGA_p5	TTTATCCGCGATCTAGGCAT	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.6	58.1	916-924 bp	1.0	69.5