Hotspot

tRNA-UUC-tRNA-GGU

LSC · 33440-34448 · 1009 bp

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Pi 0.0147

MarkerSeek score 46.20

Primer available yes

Label tRNA-UUC-tRNA-GGU

Feature Metadata

label_name: tRNA-UUC-tRNA-GGU
feature_type: igs
region: LSC
length_bp: 1009
variable_sites: 33
pi: 0.0147
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-UUC-tRNA-GGU_p1	TCGGGCATTTCTGGAAAACA	GAACCGATGACTTACGCCTT	45.0	50.0	58.0	58.0	1240-1392 bp	1.0	86.1
tRNA-UUC-tRNA-GGU_p2	CGTATCGGGCATTTCTGGAA	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	1244-1396 bp	1.0	85.8
tRNA-UUC-tRNA-GGU_p3	TTTCTCGCCGTGTTGTAACA	GAACCGATGACTTACGCCTT	45.0	50.0	58.1	58.0	1563-1712 bp	1.0	85.5
tRNA-UUC-tRNA-GGU_p4	TGGATCGGCGAATTATTGGG	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	1198-1350 bp	1.0	85.4
tRNA-UUC-tRNA-GGU_p5	TCGGGCATTTCTGGAAAACA	TACCGCTGAGTTAAAAGGGC	45.0	50.0	58.0	57.9	1205-1357 bp	1.0	85.3