Hotspot

psbM-tRNA-GUC

LSC · 31596-32725 · 1130 bp

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Pi 0.00859

MarkerSeek score 50.30

Primer available yes

Label psbM-tRNA-GUC

Feature Metadata

label_name: psbM-tRNA-GUC
feature_type: igs
region: LSC
length_bp: 1130
variable_sites: 25
pi: 0.00859
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-tRNA-GUC_p1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1239-1290 bp	1.0	62.2
psbM-tRNA-GUC_p2	AGGAACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.4	58.5	1246-1297 bp	1.0	59.0
psbM-tRNA-GUC_p3	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	55.8	57.0	1240-1291 bp	1.0	58.0
psbM-tRNA-GUC_p4	AGAATGAACAGTGCAGTAGC	TGCTATACACTTCCCTGGGA	45.0	50.0	55.8	57.4	1265-1269 bp	0.555556	40.3
psbM-tRNA-GUC_p5	AGGAACTAGAATGAACAGTGCA	TGCTATACACTTCCCTGGGA	40.9	50.0	57.4	57.4	1272-1276 bp	0.555556	37.2