Hotspot

tRNA-GCA-petN

LSC · 29625-30403 · 779 bp

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Pi 0.00824

MarkerSeek score 44.70

Primer available yes

Label tRNA-GCA-petN

Feature Metadata

label_name: tRNA-GCA-petN
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 15
pi: 0.00824
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	892-905 bp	1.0	80.6
tRNA-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	836-849 bp	1.0	80.5
tRNA-GCA-petN_p3	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	953-966 bp	1.0	78.8
tRNA-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	897-910 bp	1.0	78.7
tRNA-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	854-867 bp	1.0	78.3