Hotspot

atpA-atpF

LSC · 12902-12970 · 69 bp

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Pi 0.0137

MarkerSeek score 49.50

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 69
variable_sites: 2
pi: 0.0137
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	TGTTTGGGGCGATGAAAGAA	55.0	45.0	58.1	58.0	208-215 bp	1.0	82.6
atpA-atpF_p2	GTCGCCTACTTGAAGTACGG	TGTTTGGGGCGATGAAAGAA	55.0	45.0	58.1	58.0	206-213 bp	1.0	82.6
atpA-atpF_p3	GTCGCCTACTTGAAGTACGG	AAGGAGCTCTAGGAACCCTG	55.0	55.0	58.1	58.1	281-288 bp	1.0	80.8
atpA-atpF_p4	GTCGCCTACTTGAAGTACGG	CAAGGAGCTCTAGGAACCCT	55.0	55.0	58.1	58.1	282-289 bp	1.0	80.8
atpA-atpF_p5	CCGTCGCCTACTTGAAGTAC	AAGGAGCTCTAGGAACCCTG	55.0	55.0	58.1	58.1	283-290 bp	1.0	80.7