Hotspot

tRNA-UUU-rps16

LSC · 4345-5294 · 950 bp

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Pi 0.00439

MarkerSeek score 51.20

Primer available yes

Label tRNA-UUU-rps16

Feature Metadata

label_name: tRNA-UUU-rps16
feature_type: igs
region: LSC
length_bp: 950
variable_sites: 9
pi: 0.00439
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-UUU-rps16_p1	CCGATGGTATGGACGAATCC	GGTGCTCAACCTACAGGAAC	55.0	55.0	57.9	58.2	1114-1132 bp	1.0	81.0
tRNA-UUU-rps16_p2	CCGATGGTATGGACGAATCC	AGAAGGCGGAGGTTTTTACG	55.0	50.0	57.9	58.2	1077-1095 bp	1.0	81.0
tRNA-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	1052-1070 bp	1.0	80.5
tRNA-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	AGAAGGCGGAGGTTTTTACG	50.0	50.0	58.2	58.2	1015-1033 bp	1.0	80.5
tRNA-UUU-rps16_p5	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	1053-1071 bp	1.0	80.4