Hotspot

tRNA-GUG-psbA

LSC · 87-461 · 375 bp

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Pi 0.0173

MarkerSeek score 49.70

Primer available yes

Label tRNA-GUG-psbA

Feature Metadata

label_name: tRNA-GUG-psbA
feature_type: igs
region: LSC
length_bp: 375
variable_sites: 16
pi: 0.0173
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCGAAGCTCCATCTACAAATGG	55.0	45.5	58.1	58.2	416-441 bp	1.0	66.8
tRNA-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CGAAGCTCCATCTACAAATGG	55.0	47.6	58.1	56.8	415-440 bp	1.0	66.6
tRNA-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	TCGAAGCTCCATCTACAAATGG	50.0	45.5	57.9	58.2	430-455 bp	1.0	66.5
tRNA-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	TCGAAGCTCCATCTACAAATGG	50.0	45.5	57.9	58.2	429-454 bp	1.0	66.5
tRNA-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	TCGAAGCTCCATCTACAAATGG	50.0	45.5	57.9	58.2	431-456 bp	1.0	66.5