Hotspot

ndhF-rpl32

SSC · 111964-112751 · 788 bp

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Pi 0.0944

MarkerSeek score 81.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 788
variable_sites: 251
pi: 0.0944
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCCGAAAACCACATAGCTA	CTTTTTGACTGTCCGGTGGA	50.0	50.0	58.0	58.0	983-1244 bp	0.842105	85.1
ndhF-rpl32_p2	AAGGGGAAATTGTGCGGATT	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1553-1814 bp	0.842105	84.9
ndhF-rpl32_p3	AGGGGAAATTGTGCGGATTT	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1552-1813 bp	0.842105	84.9
ndhF-rpl32_p4	AGATTGGCCGAAAACCACAT	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	988-1249 bp	0.842105	84.8
ndhF-rpl32_p5	AGCAAGTGCACCGACAAATA	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1532-1793 bp	0.842105	84.5