Hotspot

trnT-GGU-psbD

LSC · 31086-32058 · 973 bp

Back to Liparis

Pi 0.0437

MarkerSeek score 81.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 973
variable_sites: 164
pi: 0.0437
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGTTACGAATCCCCCATCAC	GCCTAAGACTCCAGCAACTC	55.0	55.0	58.0	58.0	1819-1942 bp	0.947368	89.5
trnT-GGU-psbD_p2	GGTTCAGGTTACGAATCCCC	CTGCTACACCAAAACTGGGT	55.0	50.0	58.0	58.0	1736-1859 bp	0.894737	87.2
trnT-GGU-psbD_p3	GTTCAGGTTACGAATCCCCC	CTGCTACACCAAAACTGGGT	55.0	50.0	58.0	58.0	1735-1858 bp	0.894737	87.2
trnT-GGU-psbD_p4	GGTTCAGGTTACGAATCCCC	GCCTAAGACTCCAGCAACTC	55.0	55.0	58.0	58.0	1825-1948 bp	0.894737	86.9
trnT-GGU-psbD_p5	GTTCAGGTTACGAATCCCCC	GCCTAAGACTCCAGCAACTC	55.0	55.0	58.0	58.0	1824-1947 bp	0.894737	86.9