Hotspot

trnE-UUC-trnT-GGU

LSC · 30032-31013 · 982 bp

Back to Liparis

Pi 0.0532

MarkerSeek score 88.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 982
variable_sites: 217
pi: 0.0532
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 88.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1047-1413 bp	1.0	90.8
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	1066-1432 bp	1.0	90.6
trnE-UUC-trnT-GGU_p3	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	1026-1392 bp	0.947368	88.2
trnE-UUC-trnT-GGU_p4	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1069 bp	0.052632	40.9
trnE-UUC-trnT-GGU_p5	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	1048 bp	0.052632	40.4