Hotspot

trnK-UUU-rps16

LSC · 4300-4775 · 476 bp

Back to Liparis

Pi 0.106

MarkerSeek score 74.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 476
variable_sites: 150
pi: 0.106
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.2	57.9	640-794 bp	1.0	89.5
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.2	57.9	639-793 bp	1.0	89.5
trnK-UUU-rps16_p3	TAAGAAGGGAAGCTCGTTGC	TCGATCCCGAAGAGAAGGAA	50.0	50.0	57.9	57.9	946-1112 bp	0.947368	88.0
trnK-UUU-rps16_p4	CATCCGTCTACAATCCGCAA	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.1	57.9	1192 bp	0.052632	40.5
trnK-UUU-rps16_p5	CATCCGTCTACAATCCGCAA	GCTCAACCCACAGGAATTGT	50.0	50.0	58.1	58.4	1083 bp	0.052632	38.5