Hotspot

matK-trnK-UUU

LSC · 3193-4262 · 1070 bp

Back to Liparis

Pi 0.0612

MarkerSeek score 83.90

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1070
variable_sites: 234
pi: 0.0612
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCGCGACGAGAATGGAATTT	ATCCGGGTTGCTAACTCAAC	45.0	50.0	58.0	57.9	1278-1481 bp	0.684211	78.5
matK-trnK-UUU_p2	TCGCGACGAGAATGGAATTT	TCGATCCCGAAGAGAAGGAA	45.0	50.0	58.0	57.9	1968-2163 bp	0.684211	78.2
matK-trnK-UUU_p3	TCGCGACGAGAATGGAATTT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	58.0	58.2	1260-1463 bp	0.684211	78.0
matK-trnK-UUU_p4	AAATCGCGACGAGAATGGAA	ATCCGGGTTGCTAACTCAAC	45.0	50.0	58.0	57.9	1281-1477 bp	0.368421	64.3
matK-trnK-UUU_p5	AAATCGCGACGAGAATGGAA	TCGATCCCGAAGAGAAGGAA	45.0	50.0	58.0	57.9	1971-2166 bp	0.368421	64.1