Hotspot

rpl23

IRa · 149996-150277 · 282 bp

Back to Linum

Pi 0.274
MarkerSeek score 68.30
Primer available yes
Label rpl23

Feature Metadata

label_name
rpl23
feature_type
gene
region
IRa
length_bp
282
variable_sites
128
pi
0.274
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23_p1 GAATGGTTAAAGCGCCCAAC CACTTGACTGTCTACGGCTC 50.0 55.0 58.0 58.0 593 bp 0.4 56.1
rpl23_p2 GAATGGTTAAAGCGCCCAAC CAATTCGCCTCTGACCATCA 50.0 50.0 58.0 58.0 1231 bp 0.4 55.9
rpl23_p3 GAATGGTTAAAGCGCCCAAC CGCCTCTGACCATCAAATGA 50.0 50.0 58.0 58.0 1226 bp 0.4 55.9
rpl23_p4 GAATGGTTAAAGCGCCCAAC TCGCCTCTGACCATCAAATG 50.0 50.0 58.0 58.0 1227 bp 0.4 55.9
rpl23_p5 GAATGGTTAAAGCGCCCAAC TCTGTCGGTGCTTGAAACAA 50.0 45.0 58.0 58.0 1157 bp 0.4 55.9